epithelial marker human mouse novus nb100 687 ab 2265512 1 173yb epcam polyclonal epithelial marker human r d systems af960 ab 355745 2 112cd muc  (Novus Biologicals)

Journal: Biology

Article Title: Caco2/HT-29 In Vitro Cell Co-Culture: Barrier Integrity, Permeability, and Tight Junctions’ Composition During Progressive Passages of Parental Cells

doi: 10.3390/biology14030267

Figure Lengend Snippet: Antibodies and protocols for indirect immunofluorescence analysis. CLDN2: claudin 2; MUC-2: mucin 2. RT: room temperature; NGS: Normal Goat Serum; PBS: Phosphate-Buffered Saline; BSA: Bovine Serum Albumin.

Article Snippet: Primary antibody , Mouse anti-human claudin 2 (Invitrogen, Monza (MB), Italy, #32-5600) 1:100 in PBS 0.1 M pH 7.4 for 4 h at RT , Rabbit anti-human occludin (Invitrogen, Monza (MB), Italy, #MA3-012) 1:100 in PBS 0.1 M pH 7.4 overnight at 4 °C , Mouse anti-human MUC-2 Merck Italy (Milan, Italy, #1412598) 1:10 in 3% BSA/PBS 0.1 M pH 7.4 for 1 h at 37 °C.

Techniques: Immunofluorescence, Saline, Blocking Assay, Binding Assay