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Image Search Results
Journal: Biology
Article Title: Caco2/HT-29 In Vitro Cell Co-Culture: Barrier Integrity, Permeability, and Tight Junctions’ Composition During Progressive Passages of Parental Cells
doi: 10.3390/biology14030267
Figure Lengend Snippet: Antibodies and protocols for indirect immunofluorescence analysis. CLDN2: claudin 2; MUC-2: mucin 2. RT: room temperature; NGS: Normal Goat Serum; PBS: Phosphate-Buffered Saline; BSA: Bovine Serum Albumin.
Article Snippet: Primary antibody , Mouse anti-human claudin 2 (Invitrogen, Monza (MB), Italy, #32-5600) 1:100 in PBS 0.1 M pH 7.4 for 4 h at RT , Rabbit anti-human occludin (Invitrogen, Monza (MB), Italy, #MA3-012) 1:100 in PBS 0.1 M pH 7.4 overnight at 4 °C ,
Techniques: Immunofluorescence, Saline, Blocking Assay, Binding Assay